Immunofixation electrophoresis kits IFEU

Principle and Methodology

The IFE technique   combines zone electrophoresis with immunoprecipitation and is easier and more practical than other techniques. By IFE proteins of sample are first according to their charge separated by electrophoresis on the agarose film, and then they (as antigen) are fixed with monospecific polyclonal antisera. The monoclonal paraproteins can be heavy chain of IgG, IgA, IgM, ( IgD, IgE) and or light chain of kappa or lambda.

If  specific antigen (heavy and or light chain globulins) is present, a characteristic immunoprecipitin band will be formed, which is visualized by staining of the gel with Amido Black.

Immunofixation electrophoresis kit for manual system :

• HELLABIO IFEU01 for 10 tests
• HELLABIO IFEUD01 for 20 tests