ELECTROPHORESIS IN AGAROSE GELS REMAINS THE BASIC AND REFERENCE TECHNIQUE IN CLINICAL USE

AGAROSE VS CAPILLARY ELECTROPHORESIS


To get correct and reliable results in any diagnostic technique, much more in electrophoresis, the Specificity is as important as the Sensitivity. 

Agarose electrophoresis ensures higher specificity (98.9%) than Capillary (93.7).

 Lissoir B et al (2003): Ann Biol Clin (Paris).;61(5):557-62

 Christopher R. McCudden, PhD et al: Am J Clin Pathol 2008;129:451-458 

 

Also, paraprotein classification, which compared the CZE immunosubtraction method to immunofixation electrophoresis (IFE) on agarose, showed 100% agreement. Certain dysproteinemias involving beta lipoprotein were in partial concordance due to the inability of the CZE procedure to detect this component. 

Carl R. Jolliff and Cynthia R. Blessum (1997): ELECTROPHORESIS,18, 1781–1784

Yvonne Henskens et al (1998): Clinical Chemistry, 44, 6, 1184-1190  

Zhaohai Yang,et al (2007): Am J Clin Pathol 128(2):293-9






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