Hellabio offers IFE gels for 10 and for 20 tests

Immunofixation electrophoresis kits


 

Principle and Methodology

The IFE technique   combines zone electrophoresis with immunoprecipitation and is easier and more practical than other techniques. By IFE proteins of sample are first according to their charge separated by electrophoresis on the agarose film, and then they (as antigen) are fixed with monospecific polyclonal antisera. The monoclonal paraproteins can be heavy chain of IgG, IgA, IgM, ( IgD, IgE) and or light chain of kappa or lambda.

If  specific antigen (heavy and or light chain globulins) is present, a characteristic immunoprecipitin band will be formed, which is visualized by staining of the gel with Amido Black.

 

HELLABIO offers two kind of Immunofixation electrophoresis kit :

  • HELLABIO IFE 01 for 10 tests
  • HELLABIO IFE D01 for 20 tests
  • HELLABIO IFEU for 10 tests

 

 

 IFE 01 / IFEU

 IFE D01

 

Kit content:

 

 

 

 

 

 

10 Agarose Gels / 10 tests                          

 Electrophoresis Buffer (concentrated)

 Staining solution (concentrated)

 Destaining solution

 Protein diluent solution

 Sample templates

 Antisera templates

 Gel blotter strips

 Gel blotter sheets

 Drying blotter sheets

 Antisera  (Goat anti-Human IgG, IgA, IgM,                     -k and –λ chain)

 Fixation solution

 Instructions for Use

 

 

10 Agarose Gels / 20 tests                          

 Electrophoresis Buffer (concentrated)

 Staining solution (concentrated)

 Destaining solution

 Protein diluent solution

 Sample templates

 Antisera templates

 Gel blotter strips

 Gel blotter sheets

 Drying blotter sheets

 Antisera  (Goat anti-Human IgG, IgA, IgM,                 -k and –λ chain)

 Fixation solution

 Instructions for Use

 

 

 





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