Principle and Methodology
The IFE technique combines zone electrophoresis with immunoprecipitation and is easier and more practical than other techniques. By IFE proteins of sample are first according to their charge separated by electrophoresis on the agarose film, and then they (as antigen) are fixed with monospecific polyclonal antisera. The monoclonal paraproteins can be heavy chain of IgG, IgA, IgM, ( IgD, IgE) and or light chain of kappa or lambda.
If specific antigen (heavy and or light chain globulins) is present, a characteristic immunoprecipitin band will be formed, which is visualized by staining of the gel with Amido Black.
Immunofixation electrophoresis kit for manual system :
- HELLABIO IFE01 for 10 tests
- HELLABIO IFED01 for 20 tests
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IFE01
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IFED01
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Kit contents:
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10 agarose gels / 10 tests
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10 agarose gels / 20 tests
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Diluent solution
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Diluent solution
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Electrophoresis buffer
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Electrophoresis buffer
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Staining solution
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Staining solution
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Destaining solution
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Destaining solution
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Sample templates
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Sample templates
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Antisera templates
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Antisera templates
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Gel blotter strips
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Gel blotter strips
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Gel blotter sheets
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Gel blotter sheets
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Gel drying sheets
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Gel drying sheets
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Protein fixation solution
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Protein fixation solution
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Antisera kit
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Antisera kit
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Instructions for use
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Instructions for use
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Immunofixation electrophoresis kit for automatic system (HEELECS-1):
- HELLABIO IFEA01 for 10 tests
- HELLABIO IFEAD01 for 20 tests
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IFEA01
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IFEAD01
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Kit contents:
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10 agarose gels/10 tests
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10 agarose gels/20 tests
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Diluent solution
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Diluent solution
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Electrophoresis buffer
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Electrophoresis buffer
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Staining solution
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Staining solution
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Destaining solution
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Destaining solution
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Sample templates
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Sample templates
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Antisera templates
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Antisera templates
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Gel blotter strips
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Gel blotter strips
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Gel blotter sheets
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Gel blotter sheets
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Gel drying sheets
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Gel drying sheets
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Protein fixation solution
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Protein fixation solution
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Antisera kit
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Antisera kit
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Sponges
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Sponges
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Instructions for use |
Instructions for use |
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