Principle and Methodology
The determination of serum lipoproteins has been unitized in studies on the genesis of atherosclerosis, response to hormonal levels, congenital and developing anomalies and genotypes. Lipoproteins differ in molecular size and because of their negative charge, at ph 8,6 , they migrate, in an electrical field, toward the anode. Electrophoresis is a simple and useful technique for the evaluation of lipoprotein abnormalities and coronary disease risk factors in serum.
Agarose gel electrophoresis, in comparison to other techniques, requires less time for completion and is very useful because the migration rate of the lipoproteins is much more reproducible from run to run.
HELLABIO Agarose Gels enable the separation of lipoproteins in chylomicron, β-, pre-β-, (a) and α-lipoproteins.
Hellabio offers two kind of Lipoprotein electrophoresis kit:
HELLABIO LE10 for 10 samples / 100 tests
HELLABIO MLE for 3 samples / 48 tests
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LE10
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MLE
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Kit content
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10 Agarose Gels / 100 tests
Electrophoresis Buffer (concentrated)
Staining solution* (stock solution)
NaOH
Sample templates
Gel blotter strips
Instructions for Use
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16 Agarose Gels / 48 tests
Electrophoresis Buffer (concentrated)
Staining solution* (stock solution)
NaOH
Sample templates
Gel blotter strips
Instructions for Use
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* Fat red or Sudan black staining solution